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KMID : 1007520100190061655
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Food Science and Biotechnology
2010 Volume.19 No. 6 p.1655 ~ p.1659
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Development and Application of a Rapid and Simple Loop-mediated Isothermal Amplification Method for Food-borne Salmonella Detection
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Zhao Xihong
Wang Li
Chu Jin
Li Yanmei
Li Yanyan
Xu Zhenbo
Li Lin
Mark E. Shirtliff
He Xiaowei
Liu Yao
Wang Jihua
Yang Liansheng
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Abstract
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A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65oC for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.
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KEYWORD
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loop-mediated isothermal amplification (LAMP), Salmonella, rapid detection, food-borne pathogen, invA
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